Researchers at SmithLab_UofL presented findings at PRISMBangkok using PacBio technology for IGH genotyping, revealing high novel variation with 127 coding alleles. Novel IGH sequencing of myasthenia gravis individuals showed 86% novel alleles not in IMGT, implicating IGHG3 hinge variation. Different IGH variants can elicit varied vaccine responses. At PRISMBangkok, Sohn discussed DMR-associated isoform expression using PacBio technology. Dutta highlighted the benefits of resequencing with PacBio for identifying complex rearrangements. PacBio announced a genomics consortium in Asia to study subfertility diagnostics. Henry achieved a T2T PacBio HiFi genome of mango without additional tech. Pond emphasized the importance of early detection of rare TB mutations using PacBio Onso sequencing. LSHTM utilized Nanopore technology for accurate AMR detection and resolving plasmids in Klebsiella pneumoniae.
🚀🧬Our first long-read #HiFi human genomes have been sequenced in #Rouen, France! Blown away by the data quality and all-inclusive bioinformatics pipeline producing phased, methylation resolved data & a powerfull detection of all types of variants 🤯 @PacBio @GJRNicolas https://t.co/wQwQlJ13lw
High-quality means complete. Short-read sequencing cannot accurately delineate AMR genes. Read how the team at @LSHTM used #nanopore sequencing to resolve plasmids & mobile elements in Klebsiella pneumoniae, overcoming previous limitations. Read here: https://t.co/OgHuJO10JD https://t.co/gQmOrYDdpA
Pond: early identification of rare TB mutation important for managing antibiotic resistance. Deep @PacBio Onso seq of MRD gene amplicons detects minor variants down to 0.001% at 100k reads for katG. #PRISMBangkok
Henry: T2T @PacBio HiFi genome of mango without needing Hi-C or other tech …! #PRISMBangkok https://t.co/SJV3C48VeQ
Exciting update from #PacBioPRISM in Bangkok! We’ve signed a memorandum of understanding (MOU) for a human genomics consortium with 5 centers across Asia. 🌏🔬 Together, we’ll study the diagnostic journey of individuals with subfertility using PacBio HiFi long-read sequencing. https://t.co/tXPSH078lu
Dutta: WGS w ONT resulted in low coverage and difficulty interpreting. Resequence with @PacBio identified complex rearrangement across chr 4-5-6. Clean data makes identifying breakpoints and indels easier. #PRISMBangkok
Inaccurate #AMR detection hampers public health efforts. Read how @LSHTM used #nanopore technology to overcome current challenges of identifying resistant markers, providing a rapid and robust method for AMR surveillance. Learn more: https://t.co/BzKAkGb4Oi #WYMM https://t.co/8yXN8HCaVt
Sohn: @PacBio MAS/Kinnex (I think bulk not since cell) data of AK1-NPC-iPSC finds DMR-associated specific isoform expression - linking methylation patterns to transcription. #PRISMBangkok
.@SmithLab_UofL : DNA and RNA IGH @PacBio sequencing of myasthenia gravis (MG) individuals allows antibody sub-isotype analysis. 86% of novel alleles NOT in IMGT. IGHG3 hinge variation implicated. #PRISMBangkok
.@SmithLab_UofL : IGH variants often vary by single base, yet one allele to another might solicit completely different vaccine responses. @PacBio #PRISMBangkok
.@SmithLab_UofL : novel IGH genotyping using targeted @PacBio HiFi of 10-15kb fragments, QV40+. Largest dataset to date show high novel variation. 127 novel coding alleles. https://t.co/chJEBSIpDb